MVPrepTMPlasmid Kit

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MVPrepTMPlasmid Kit

Max. 30ug of Plasmid DNA Isolation from up to 10 ml Cultures

  • High and Low Copy Plasmid Isolation from 1-10 ml of cultures
  • Endonuclease positive stains washing buffer included
  • ~20 minutes Spin Column based protocol

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Description

Item Overview
The MVPrepTM Plasmid Kit is devised for the rapid and convenient isolation of Plasmid DNA from cultured gram-negative bacteria. With its optimized buffer and straightforward experimental procedure, this kit enables the reliable isolation of high-purity plasmid DNA, up to 20 kbp, in quantities of up to 20-30 µg. The isolated plasmid, characterized by its high purity, can be applied to various downstream experiments, including gene cloning, sequencing, and enzymatic restriction analysis, and more.
Key Attributes

  • Endonuclease positive stains washing buffer included.
  • Reliable isolation of high-purity plasmid DNA, up to 20 kbp.
  • A straightforward experimental procedure with numerical buffer names minimizes human error.
Applications and uses

  • PCR
  • RT-PCR
  • Sequencing
  • Northern Blotting
  • Primer extension
  • Other downstream application

Technical Specs
Kit Components
Cat No.DP-40050R / DP-40100R / DP-40200R
Prep No.50 Preps / 100 Preps / 100 Preps x 2
Spin Column(Collection Tube included, 50 ea / pk)1pk / 2pk / 2pk x 2
Buffer P0115 mL / 30 mL / 30 mL x 2
Buffer P0215 mL / 30 mL / 30 mL x 2
Buffer P0320 mL / 40 mL / 40 mL x 2
Buffer P04* (Add Ethanol)17.5 mL / 35 mL / 35 mL x 2
Buffer P05* (Add Ethanol)8 mL / 16 mL / 16 mL x 2
Buffer P0615 mL / 15 mL / 15 mL x 2
RNase A**, ***(Lyophillized)1 ea / 1 ea / 1 ea x 2
Protocol1 ea / 1 ea / 1 ea x 2
Technical Specifications
FeaturesMVPrepTM Plasmid Kit
ApplicationsGene modification, Sequencin, etc.
Processing typeSilica-membrane based Column
Starting Sample Volume1-5 ml for high copy / 6-10 ml for low copy
Starting Sample SrainEndonuclease negative stains, Endonuclease positive stains
Plasmid TypeHigh copy plasmid, Low copy plasmid
Processing Time~20 min
Recommended Elution Volume30-50 ul
Yield<20 ug
Workflow & One Page Protocol

Workflow of Plasmid Kit
Sample Text

Performance Evaluation

Performance Evaluation of Plasmid Kit
Sample Text

Manuals/Documents

User Manual

Brochure

Supporting Documents

Ordering Information
Modification (Mod.) Name5' Mod.3' Mod.Internal Mod.
Acrydite
done
Aldehyde Modifier (CHO)
done
Alkyne Modifier
done

done
Amino Modifier - NH2 C12
done

done

Please login to post questions

Description

Custom description tab.

Quantity Pricing

Here's your quantity pricing tab.

Other Products

Here's your other products tab.

Description

Item Overview
The MVPrepTM Plasmid Kit is devised for the rapid and convenient isolation of Plasmid DNA from cultured gram-negative bacteria. With its optimized buffer and straightforward experimental procedure, this kit enables the reliable isolation of high-purity plasmid DNA, up to 20 kbp, in quantities of up to 20-30 µg. The isolated plasmid, characterized by its high purity, can be applied to various downstream experiments, including gene cloning, sequencing, and enzymatic restriction analysis, and more.
Key Attributes

  • Endonuclease positive stains washing buffer included.
  • Reliable isolation of high-purity plasmid DNA, up to 20 kbp.
  • A straightforward experimental procedure with numerical buffer names minimizes human error.
Applications and uses

  • PCR
  • RT-PCR
  • Sequencing
  • Northern Blotting
  • Primer extension
  • Other downstream application

Technical Specs
Kit Components
Cat No.DP-40050R / DP-40100R / DP-40200R
Prep No.50 Preps / 100 Preps / 100 Preps x 2
Spin Column(Collection Tube included, 50 ea / pk)1pk / 2pk / 2pk x 2
Buffer P0115 mL / 30 mL / 30 mL x 2
Buffer P0215 mL / 30 mL / 30 mL x 2
Buffer P0320 mL / 40 mL / 40 mL x 2
Buffer P04* (Add Ethanol)17.5 mL / 35 mL / 35 mL x 2
Buffer P05* (Add Ethanol)8 mL / 16 mL / 16 mL x 2
Buffer P0615 mL / 15 mL / 15 mL x 2
RNase A**, ***(Lyophillized)1 ea / 1 ea / 1 ea x 2
Protocol1 ea / 1 ea / 1 ea x 2
Technical Specifications
FeaturesMVPrepTM Plasmid Kit
ApplicationsGene modification, Sequencin, etc.
Processing typeSilica-membrane based Column
Starting Sample Volume1-5 ml for high copy / 6-10 ml for low copy
Starting Sample SrainEndonuclease negative stains, Endonuclease positive stains
Plasmid TypeHigh copy plasmid, Low copy plasmid
Processing Time~20 min
Recommended Elution Volume30-50 ul
Yield<20 ug
Workflow & One Page Protocol

Workflow of Plasmid Kit
Sample Text

Performance Evaluation

Performance Evaluation of Plasmid Kit
Sample Text

Manuals/Documents

User Manual

Brochure

Supporting Documents

Ordering Information
Modification (Mod.) Name5' Mod.3' Mod.Internal Mod.
Acrydite
done
Aldehyde Modifier (CHO)
done
Alkyne Modifier
done

done
Amino Modifier - NH2 C12
done

done

Please login to post questions

Description

Custom description tab.

Quantity Pricing

Here's your quantity pricing tab.

Other Products

Here's your other products tab.

Description

Item Overview
The MVPrepTM Plasmid Kit is devised for the rapid and convenient isolation of Plasmid DNA from cultured gram-negative bacteria. With its optimized buffer and straightforward experimental procedure, this kit enables the reliable isolation of high-purity plasmid DNA, up to 20 kbp, in quantities of up to 20-30 µg. The isolated plasmid, characterized by its high purity, can be applied to various downstream experiments, including gene cloning, sequencing, and enzymatic restriction analysis, and more.
Key Attributes

  • Endonuclease positive stains washing buffer included.
  • Reliable isolation of high-purity plasmid DNA, up to 20 kbp.
  • A straightforward experimental procedure with numerical buffer names minimizes human error.
Applications and uses

  • PCR
  • RT-PCR
  • Sequencing
  • Northern Blotting
  • Primer extension
  • Other downstream application

Technical Specs
Kit Components
Cat No.DP-40050R / DP-40100R / DP-40200R
Prep No.50 Preps / 100 Preps / 100 Preps x 2
Spin Column(Collection Tube included, 50 ea / pk)1pk / 2pk / 2pk x 2
Buffer P0115 mL / 30 mL / 30 mL x 2
Buffer P0215 mL / 30 mL / 30 mL x 2
Buffer P0320 mL / 40 mL / 40 mL x 2
Buffer P04* (Add Ethanol)17.5 mL / 35 mL / 35 mL x 2
Buffer P05* (Add Ethanol)8 mL / 16 mL / 16 mL x 2
Buffer P0615 mL / 15 mL / 15 mL x 2
RNase A**, ***(Lyophillized)1 ea / 1 ea / 1 ea x 2
Protocol1 ea / 1 ea / 1 ea x 2
Technical Specifications
FeaturesMVPrepTM Plasmid Kit
ApplicationsGene modification, Sequencin, etc.
Processing typeSilica-membrane based Column
Starting Sample Volume1-5 ml for high copy / 6-10 ml for low copy
Starting Sample SrainEndonuclease negative stains, Endonuclease positive stains
Plasmid TypeHigh copy plasmid, Low copy plasmid
Processing Time~20 min
Recommended Elution Volume30-50 ul
Yield<20 ug
Workflow & One Page Protocol

Workflow of Plasmid Kit
Sample Text

Performance Evaluation

Performance Evaluation of Plasmid Kit
Sample Text

Manuals/Documents

User Manual

Brochure

Supporting Documents

Ordering Information
Modification (Mod.) Name5' Mod.3' Mod.Internal Mod.
Acrydite
done
Aldehyde Modifier (CHO)
done
Alkyne Modifier
done

done
Amino Modifier - NH2 C12
done

done

Please login to post questions

Description

Custom description tab.

Quantity Pricing

Here's your quantity pricing tab.

Other Products

Here's your other products tab.

Associated Items

  • Sample – MV Other

     Others
    MVPrepTM Blood gDNA Kit
    • Suitable for PCR, RT-PCR, or any downstream application
    • Contains all required consumables such as spin column and elution tube
    • Long term storage condition(room temperature (15~25℃) maximum of 12 months)
    • Ensure high yield and consistent result
    • Easy-to-use …

  • Sample – Labor other

     Others
    MVPrepTM Blood gDNA Kit
    • Suitable for PCR, RT-PCR, or any downstream application
    • Contains all required consumables such as spin column and elution tube
    • Long term storage condition(room temperature (15~25℃) maximum of 12 months)
    • Ensure high yield and consistent result
    • Easy-to-use …

  • Sample – R/D Amp_other

     Others
    MVPrepTM Blood gDNA Kit
    • Suitable for PCR, RT-PCR, or any downstream application
    • Contains all required consumables such as spin column and elution tube
    • Long term storage condition(room temperature (15~25℃) maximum of 12 months)
    • Ensure high yield and consistent result
    • Easy-to-use …

  • Sample – PREMADE_other

     Others
    MVPrepTM Blood gDNA Kit
    • Suitable for PCR, RT-PCR, or any downstream application
    • Contains all required consumables such as spin column and elution tube
    • Long term storage condition(room temperature (15~25℃) maximum of 12 months)
    • Ensure high yield and consistent result
    • Easy-to-use …

Description

Item Overview
The MVPrepTM Plasmid Kit is devised for the rapid and convenient isolation of Plasmid DNA from cultured gram-negative bacteria. With its optimized buffer and straightforward experimental procedure, this kit enables the reliable isolation of high-purity plasmid DNA, up to 20 kbp, in quantities of up to 20-30 µg. The isolated plasmid, characterized by its high purity, can be applied to various downstream experiments, including gene cloning, sequencing, and enzymatic restriction analysis, and more.
Key Attributes

  • Endonuclease positive stains washing buffer included.
  • Reliable isolation of high-purity plasmid DNA, up to 20 kbp.
  • A straightforward experimental procedure with numerical buffer names minimizes human error.
Applications and uses

  • PCR
  • RT-PCR
  • Sequencing
  • Northern Blotting
  • Primer extension
  • Other downstream application

Technical Specs
Kit Components
Cat No.DP-40050R / DP-40100R / DP-40200R
Prep No.50 Preps / 100 Preps / 100 Preps x 2
Spin Column(Collection Tube included, 50 ea / pk)1pk / 2pk / 2pk x 2
Buffer P0115 mL / 30 mL / 30 mL x 2
Buffer P0215 mL / 30 mL / 30 mL x 2
Buffer P0320 mL / 40 mL / 40 mL x 2
Buffer P04* (Add Ethanol)17.5 mL / 35 mL / 35 mL x 2
Buffer P05* (Add Ethanol)8 mL / 16 mL / 16 mL x 2
Buffer P0615 mL / 15 mL / 15 mL x 2
RNase A**, ***(Lyophillized)1 ea / 1 ea / 1 ea x 2
Protocol1 ea / 1 ea / 1 ea x 2
Technical Specifications
FeaturesMVPrepTM Plasmid Kit
ApplicationsGene modification, Sequencin, etc.
Processing typeSilica-membrane based Column
Starting Sample Volume1-5 ml for high copy / 6-10 ml for low copy
Starting Sample SrainEndonuclease negative stains, Endonuclease positive stains
Plasmid TypeHigh copy plasmid, Low copy plasmid
Processing Time~20 min
Recommended Elution Volume30-50 ul
Yield<20 ug
Workflow & One Page Protocol

Workflow of Plasmid Kit
Sample Text

Performance Evaluation

Performance Evaluation of Plasmid Kit
Sample Text

Manuals/Documents

User Manual

Brochure

Supporting Documents

Ordering Information
Modification (Mod.) Name5' Mod.3' Mod.Internal Mod.
Acrydite
done
Aldehyde Modifier (CHO)
done
Alkyne Modifier
done

done
Amino Modifier - NH2 C12
done

done

Please login to post questions

Description

Custom description tab.

Quantity Pricing

Here's your quantity pricing tab.

Other Products

Here's your other products tab.

Q&A

각 buffer에 대해 추가 구매는 가능하지만 권장하지 않습니다. 제품 유효기간 관리도 어려워 성능이 떨어진 제품을 사용할 수도 있고, 중간에 제품 리뉴얼이 되면 구성이 변경될 수도 있습니다. Midi와Maxi 제품의 경우, buffer 추가적으로 판매하고 있지 않습니다(Column은 별도 구매 가능하지만 Midi와 Maxi 제품의 Syringe Filter는 별도 구매 불가능합니다.)

각 buffer에 대해 추가 구매는 가능하지만 권장하지 않습니다. 제품 유효기간 관리도 어려워 성능이 떨어진 제품을 사용할 수도 있고, 중간에 제품 리뉴얼이 되면 구성이 변경될 수도 있습니다. Midi와Maxi 제품의 경우, buffer 추가적으로 판매하고 있지 않습니다(Column은 별도 구매 가능하지만 Midi와 Maxi 제품의 Syringe Filter는 별도 구매 불가능합니다.)

Resuspension Buffer (예: PA1, PNA1, PM1)로 powder를 녹인 후, Resuspension buffer에 첨가하여 사용하면 됩니다. Buffer에 RNase A를 첨가한 후에는 4°C에서 보관해야 하며, 6개월 이내에 사용을 권장 드립니다. 자세한 사항은 제품 매뉴얼을 참고해주시기 바랍니다.

Notification

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